cdma

RESULTADOS

EM FASE DE ACREDITAÇÃO ISO/ IEC 17.025


Molecular Analysis of Brazilian Infectious Br onchitis Field Isolates by Reverse Transcription–Polymerase Chain Reaction, Restriction Fragment Length Polymorphism, and Partial Sequencing of the N Gene

SUMMARY. Molecular analysis of 15 Brazilian infectious bronchitis virus (IBV) isolates, obtained from clinical outbreaks of the
disease in chickens (broilers or layers) in the state of Minas Gerais (Brazil) between 1972 and 1989, is reported. Using the N protein
gene as target, IBVs were analyzed by reverse transcription–polymerase chain reaction/restriction fragment length polymorphism
(RT-PCR/RFLP) with the restriction enzymes AvaII, Hph I, Sau 96I, and Tsp 509I and cDNA sequencing. Results obtained from
those isolates were compared to 19 sequences available in GenBank. N gene RFLP profiles, cDNA sequences, and predicted amino
acid composition were used for the construction of dendrograms. Brazilian isolates were grouped into one distinct group. Identity
of predicted N protein amino acid composition varied from 45% (between isolates G and 208) up to 99% (PM1 and PM2), and,
when compared to the other IBVs, the amino acid identity was from 42% (Q3/88 and G) up to 97% (D41 and PM1). The great
genetic diversity was shown to occur before the official use of vaccination in Brazil and has remained thereafter.
 
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